Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I

LE Formosa; L Muellner-Wong; B Reljic; AJ Sharpe; TD Jackson; TH Beilharz; D Stojanovski; M Lazarou; DA Stroud; MT Ryan

Journal article

Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I

LE Formosa, L Muellner-Wong, B Reljic, AJ Sharpe, TD Jackson, TH Beilharz, D Stojanovski, M Lazarou, DA Stroud, MT Ryan

Cell Reports | CELL PRESS | Published : 2020

DOI: 10.1016/j.celrep.2020.107541

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Abstract

Formosa et al. investigate the function of the MCIA complex in complex I assembly. They demonstrate the requirement of individual components for the formation of complex I intermediates and assembly of the final enzyme. Finally, they characterize the involvement of TMEM186 and COA1 in this process.

University of Melbourne Researchers

Diana Stojanovski Author

Michael Lazarou Author

David Stroud Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

We thank lab members for constructive discussions, the Bio21 Mass Spectrometry and Proteomics Facility (MMSPF), the Monash Proteomics and Metabolomics Facility (MPMF) for the provision of instrumentation, training, and technical support, and Monash Flowcore for cell sorting. T.D.J. is supported by an Australian Government Research Training Program (RTP) Scholarship. We acknowledge funding from the National Health and Medical Research Council (NHMRC project grants 1164459 to M.T.R., 1125390 and 1140906 to M.T.R. and D.A.S., and NHMRC Fellowship 1140851 to D.A.S.), a Research Fellowship from the Mito Foundation, and funding from the Australian Research Council (DP170101249 to D.S.).